Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Yazarlar

DOI:

https://doi.org/10.26124/bec:2021-0001

Anahtar Kelimeler:

ATP- ATP production- high-resolution respirometry- Magnesium Green- mitochondria- oxidative phosphorylation- fluorometry- FluoRespirometry

Öz

For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.

Cite:

Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001

İndir

Ek Dosyalar

Yayınlanmış

2021-06-30

Sayı

Cilt 2021

Bölüm

Living Communications

Kategoriler

Lisans

Telif Hakkı (c) 2021 Luiza Helena Daltro Cardoso, Carolina Doerrier, Erich Gnaiger

Creative Commons License

Bu çalışma Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License ile lisanslanmıştır.

Nasıl Atıf Yapılır

Daltro Cardoso, L. H., Doerrier, C., & Gnaiger, E. (2021). Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenergetics Communications, 2021, 1. https://doi.org/10.26124/bec:2021-0001