Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Аутори

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https://doi.org/10.26124/bec:2021-0001

Кључне речи:

ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry

Апстракт

For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.

Cite:

Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001

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Објављено

2021-06-30

Број часописа

Год. 2021

Рубрика

Living Communications

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Лиценца

Сва права задржана (c) 2021 Luiza Helena Daltro Cardoso, Carolina Doerrier, Erich Gnaiger

Creative Commons License

Овај рад је под Creative Commons Aуторство-Nекомерцијално-Без прераде 4.0 Интернационална лиценца.

Како цитирати

Daltro Cardoso, L. H., Doerrier, C., & Gnaiger, E. (2021). Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenergetics Communications, 2021, 1. https://doi.org/10.26124/bec:2021-0001