OXPHOS coupling and uncoupling

Avtorji

DOI:

https://doi.org/10.26124/bec.2024-0005

Ključne besede:

electron transfer capacity, LEAK respiration, mitochondria, oxidative phosphorylation

Povzetek

Coupling of OXPHOS is classically studied by comparing ADP-stimulated O2 flux (OXPHOS capacity P at saturating ADP and Pi concentrations) with non-phosphorylating LEAK respiration L, maintained mainly to compensate for the proton leak at high chemiosmotic potential in mitochondrial preparations supplied with fuel substrates to support mitochondrial electron transfer. However, measurement of the non-coupled electron transfer capacity E (ET capacity at collapsed chemiosmotic potential) yields additional information on coupling, since ET capacity may exceed OXPHOS capacity.

Cite:

Cardoso LHD, Gnaiger E (2024) OXPHOS coupling and uncoupling. Bioenerg Commun 2024.5. https://doi.org/10.26124/bec.2024-0005

Prenosi

Objavljeno

2024-08-09

Številka

Letn. 2024

Rubrika

Living Communications

Kategorije

Licenca

Avtorske pravice (c) 2024 Luiza Helena Daltro Cardoso, Erich Gnaiger

Creative Commons licenca

To delo je licencirano pod Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 mednarodno licenco.

Kako citirati

Cardoso, L. H. D., & Gnaiger, E. (2024). OXPHOS coupling and uncoupling . Bioenergetics Communications, 2024, 5. https://doi.org/10.26124/bec.2024-0005