Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration
DOI:
https://doi.org/10.26124/bec:2021-0001Palavras-chave:
ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometryResumo
For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.
Cite:
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001
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Alguns direitos reservados 2021 Luiza Helena Daltro Cardoso, Carolina Doerrier, Erich Gnaiger
Este trabalho encontra-se publicado com a Licença Internacional Creative Commons Atribuição-NãoComercial-SemDerivações 4.0.
