Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration

Autor

DOI:

https://doi.org/10.26124/bec:2021-0001

Słowa kluczowe:

ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry

Abstrakt

For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.

Cite:

Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001

Pobrania

Pliki dodatkowe

Opublikowane

2021-06-30

Numer

Tom 2021

Dział

Living Communications

Kategorie

Licencja

Prawa autorskie (c) 2021 Luiza Helena Daltro Cardoso, Carolina Doerrier, Erich Gnaiger

Creative Commons License

Utwór dostępny jest na licencji Creative Commons Uznanie autorstwa – Użycie niekomercyjne – Bez utworów zależnych 4.0 Międzynarodowe.

Jak cytować

Daltro Cardoso, L. H., Doerrier, C., & Gnaiger, E. (2021). Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenergetics Communications, 2021, 1. https://doi.org/10.26124/bec:2021-0001