OXPHOS coupling and uncoupling

Auteur/ices

DOI :

https://doi.org/10.26124/bec.2024-0005

Mots-clés :

electron transfer capacity, LEAK respiration, mitochondria, oxidative phosphorylation

Résumé

Coupling of OXPHOS is classically studied by comparing ADP-stimulated O2 flux (OXPHOS capacity P at saturating ADP and Pi concentrations) with non-phosphorylating LEAK respiration L, maintained mainly to compensate for the proton leak at high chemiosmotic potential in mitochondrial preparations supplied with fuel substrates to support mitochondrial electron transfer. However, measurement of the non-coupled electron transfer capacity E (ET capacity at collapsed chemiosmotic potential) yields additional information on coupling, since ET capacity may exceed OXPHOS capacity.

Cite:

Cardoso LHD, Gnaiger E (2024) OXPHOS coupling and uncoupling. Bioenerg Commun 2024.5. https://doi.org/10.26124/bec.2024-0005

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Publiée

2024-08-09

Numéro

Vol. 2024

Rubrique

Living Communications

Catégories

Licence

Certains droits réservés 2024 Luiza Helena Daltro Cardoso, Erich Gnaiger

Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d'Utilisation Commerciale - Pas de Modification 4.0 International.

Comment citer

Cardoso, L. H. D., & Gnaiger, E. (2024). OXPHOS coupling and uncoupling . Bioenergetics Communications, 2024, 5. https://doi.org/10.26124/bec.2024-0005