OXPHOS coupling and uncoupling

Forfattere

DOI:

https://doi.org/10.26124/bec.2024-0005

Nøgleord:

electron transfer capacity, LEAK respiration, mitochondria, oxidative phosphorylation

Resumé

Coupling of OXPHOS is classically studied by comparing ADP-stimulated O2 flux (OXPHOS capacity P at saturating ADP and Pi concentrations) with non-phosphorylating LEAK respiration L, maintained mainly to compensate for the proton leak at high chemiosmotic potential in mitochondrial preparations supplied with fuel substrates to support mitochondrial electron transfer. However, measurement of the non-coupled electron transfer capacity E (ET capacity at collapsed chemiosmotic potential) yields additional information on coupling, since ET capacity may exceed OXPHOS capacity.

Cite:

Cardoso LHD, Gnaiger E (2024) OXPHOS coupling and uncoupling. Bioenerg Commun 2024.5. https://doi.org/10.26124/bec.2024-0005

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Publiceret

2024-08-09

Nummer

Årg. 2024

Sektion

Living Communications

Kategorier

Licens

Ophavsret (c) 2024 Luiza Helena Daltro Cardoso, Erich Gnaiger

Creative Commons License

Dette værk er udgivet under licensen Creative Commons Kreditering–IkkeKommerciel–IngenBearbejdelse (CC BY-NC-ND).

Citation/Eksport

Cardoso, L. H. D., & Gnaiger, E. (2024). OXPHOS coupling and uncoupling . Bioenergetics Communications, 2024, 5. https://doi.org/10.26124/bec.2024-0005