OXPHOS coupling and uncoupling

Autors/ores

DOI:

https://doi.org/10.26124/bec.2024-0005

Paraules clau:

electron transfer capacity, LEAK respiration, mitochondria, oxidative phosphorylation

Resum

Coupling of OXPHOS is classically studied by comparing ADP-stimulated O2 flux (OXPHOS capacity P at saturating ADP and Pi concentrations) with non-phosphorylating LEAK respiration L, maintained mainly to compensate for the proton leak at high chemiosmotic potential in mitochondrial preparations supplied with fuel substrates to support mitochondrial electron transfer. However, measurement of the non-coupled electron transfer capacity E (ET capacity at collapsed chemiosmotic potential) yields additional information on coupling, since ET capacity may exceed OXPHOS capacity.

Cite:

Cardoso LHD, Gnaiger E (2024) OXPHOS coupling and uncoupling. Bioenerg Commun 2024.5. https://doi.org/10.26124/bec.2024-0005

Descàrregues

Publicat

2024-08-09

Número

Vol. 2024

Secció

Living Communications

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Llicència

Drets d'autor (c) 2024 Luiza Helena Daltro Cardoso, Erich Gnaiger

Creative Commons License

Aquesta obra està sota una llicència internacional Creative Commons Reconeixement-NoComercial-SenseObraDerivada 4.0.

Com citar

Cardoso, L. H. D., & Gnaiger, E. (2024). OXPHOS coupling and uncoupling . Bioenergetics Communications, 2024, 5. https://doi.org/10.26124/bec.2024-0005